Difference between a genomic and a cDNA library

Difference between a genomic and a cDNA library:

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1. What is the difference between a genomic and a cDNA library? What library would you want to screen if you needed to find a protein-encoding gene?

2. What is the purpose of Sanger sequencing? What is the reason the ddNTPs used lack a 3’-hydroxyl group?

4. The questions below are based on RT-PCR:

a. How is mRNA purified from cells?

b. How does reverse transcriptase make cDNA? How is cDNA different that DNA?

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Instructor’s Comment

Instructors Comments:Hi Irene, Qualitative Feedback: You completed this assignment in a detailed and complete manner. You included a comparison of research in relation to methodology in a focused manner with supporting details. A comparison of research in relation to goals and data collection is well developed with supporting details included. Number of required resources is met; APA format intact. Quantitative Feedback: please see the rubric.

Irene

Instructor's Comments:Hi Irene, Your initial post answered the question and provoked further discussion. You included information and rationale to support your discussion. Relevant citations were included.

Irene2

nstructor's Comments:Hi Irene, Your initial post was detailed and included relevant information. You provided insight into our discussions. You included information and rationale to support your discussion. Appropriate citations were included.

Irene 4

Instructor's Comments:Hi Irene, Thank you! Your post was comprehensive. You answered the question with detail and provided specific examples. Sources were relevant and recent.

Irene 4

Instructor's Comments:Hi Irene, Thank you for your response! Your ideas were presented in a logical manner. Details and examples were provided to support your thoughts. Relevant resources grounded your ideas in the literature.